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Ca2+-dependent kinetics of CD39 mutants
| Km | Vmax | |
|---|---|---|
| nm[Pi]/min/μg | ||
| WT | 2.91×10-5 | 2.91 |
| Δ1 | 4.62×10-5 | 1.54 |
| Δ4 | 3.51×10-5 | 1.75 |
| Δ5 | 2.72×10-5 | 2.72 |
| Δ7 | 7.72×10-6 | 1.93 |
| +4,7 | 2.35×10-5 | 0.71 |
Concentrations of free calcium were calculated using the MaxChelator program with calcium complex constants from Martell and Smith (1974). The graphing program IGOR was used to display calcium titration curves and to calculate Km values for free Ca2+.
