Figure 3.

Localization of occludin peptides. Divalent cation depletion was used to dissemble tight junction (TJ) structures of polarized, confluent T84 cell monolayers. Bait peptide (200 μM O-A:101–121* or O-B: 210–228*) or media used for peptide addition (CTRL) was added at the time of Ca²⁺ repletion. After a 6- or 24-h incubation, monolayers were washed free of unbound peptide, fixed with 3.7% paraformaldehyde, permeabilized with 0.2% Triton X-100, and prepared for fluorescence microscopy. Distribution of bound bait peptides was determined by staining with FITC-conjugated streptavidin (green), whereas Alexa 568-phalloidin was used to highlight the F-actin architecture (red). Scale bar, 10 μm.