Figure 5.

Effects of peptides on TJ function. Divalent cation depletion was used to dissemble tight junction (TJ) structures of polarized, confluent T84 cell monolayers. (A) O-B:210–228 was added to final concentrations from 25 to 400 μM at the time of Ca²⁺ repletion. Addition of media used for peptide additions was used as a control (CTRL). Transepithelial electrical resistance (TER) measurements were performed over the next 24 h using “chopstick” electrodes. (B) After divalent cation depletion, 200 μM O-A:101–121, O-B:210–228, control (scrambled O-B:210–228 sequence) peptides, or control media was added to T84 monolayers at the time of Ca²⁺ repletion. TER measurements were made over the next 24 h using chopstick electrodes. (C) After addition of 200 μM O-A:101–121, O-B:210–228, or control media at the time of Ca²⁺ repletion, paracellular permeability was determined as a rate of 3-kDa fluorescent dextran transport at T = 0, 6, and 24 h.