Figure 5.

Colocalization of Ecad-mRFP with Rab11wt-GFP in live HeLa cells. HeLa cells, cotransfected with Rab11wt-GFP and Ecad-mRFP, were imaged live. A single image at the plane of the Golgi complex shows colocalization in some of the larger (1–3 μm), stationary structures (A). Ecad-mRFP is concentrated in the Golgi, in PGCs and in the larger structures colabeled with Rab11wt-GFP. Sequential images (cropped from dashed box in A) show colabeled intermediate compartments that move locally, whereas surrounding PGCs move on longer trajectories through this field (B). PGCs containing Rab11wt-GFP, Ecad-mRFP, or both, can be observed budding from and fusing with the intermediate compartments in the live movie sequence (Supplementary Movie 4). Scale bars, (A) 10 μm; (B) 5 μm.