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. 2005 Apr;16(4):1769–1776. doi: 10.1091/mbc.E04-08-0739

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Copyright © 2005, The American Society for Cell Biology

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Figure 1. — Levels of clathrin heavy and light chains are coordinately decreased due to siRNA-mediated decreases in clathrin heavy chain. (A and B) TRVb-EFL cells transiently transfected with CHC siRNA (n = 341) exhibited an expanded range of clathrin levels compared with cells under control conditions (n = 187). Levels of clathrin light chain (A) and clathrin heavy chain (B) were decreased as a result of CHC siRNA transfection. Clathrin light chain levels were determined via the stably expressed EGFP-clathrin light chain fusion protein and clathrin heavy-chain levels were determined via immunostaining against clathrin heavy chain. (C) The concentration of the fluorescence associated with clathrin heavy chain and clathrin light chain scale directly. The data points represent the averaged fluorescence values over several cells ± SEM. Scale bar, 10 μm.