Figure 1.
SCFMet30 mutants are cadmium sensitive. (A) Serial dilutions of cells growing in YEPD medium in mid-log phase were spotted onto YEPD plates containing cadmium at the concentrations indicated. Plates were incubated at 28°C. (B) Same as in A, but cells were grown and plates were incubated at 25°C. Strains used were met30-6 (PY283), met30-9 (PY281), cdc34-3 (PY23), cdc53-1 (PY175), and cdc4-3 (PY187). (C) Wild-type cells and met30-6 (PY283) were grown in YEPD at the indicated temperatures and expression of GSH1 was measured by real-time RT-PCR. Expression levels were normalized to expression of the ACT1 gene. (D) Wild-type cells and met30-6 mutants were grown at 28°C in YEPD incubated with various concentrations of cadmium as indicated for 30 min. Dilutions of the cells were plated on YEPD plates and incubated at 25°C. Colonies were counted after 4 d. The number of colonies after no cadmium exposure was set to 100%. Error bars denote SE of the mean derived from four data sets.