Figure 2.

Cadmium induces a Met4 and Met32 dependent cell cycle arrest. (A) Wild-type cells and met30-6 mutants (PY283) were grown at 28°C in YEPD to early-log phase, arrested with α-factor until >90% of cells were unbudded and then released from the α-factor block into media with or without 200 μM cadmium. Cell cycle progression was followed by flow cytometry. (B and C) Serial dilutions of cells growing in YEPD medium in mid-log phase at 30°C were spotted onto YEPD plates containing cadmium at the concentrations indicated. Plates were incubated at 30°C. Strains used: met4Δ (PY1123), met4Δ met30Δ (PY589), met32Δ (PY889). met32Δ met30Δ (PY893), met28Δ (PY950), met31Δ (PY965), met32Δ (PY889), met31Δ met32Δ (PY968), and cbf1Δ (PY640).