Figure 6.

Effects of various chemicals on Met4 and Rad53. (A) Wild-type cells were grown to an OD₆₀₀ = 0.5, incubated with different concentrations of the chemicals indicated for 30 min, and cell lysates were analyzed by immunoblotting with antibodies directed against Met4 or Rad53 (gel 7.5%, control anti-Rpt1). The concentrations of the chemicals were as follows: MMS, 0.01, 0.03, and 0.1%; H₂O₂, 1, 3, and 10 mM; Cd, 0.1, 0.3, and 1 mM; As, 1, 3, and 10 mM; Co and Ni, 5, 20, and 50 mM; and Pb, 0.5 and 1.5 mM. The first lane in each panel is an untreated sample.