Table 2.
Plasmids used in this study
| Plasmid | Characteristic | Source |
|---|---|---|
| pAS24 | YCplac111, CEN, LEU2, allows expression of proteins N-terminally tagged with a double HA-tag under the control of the GAL1 promoter | Schmidt et al. (1997) |
| pAS25 | YCplac33, CEN, URA3, allows expression of proteins N-terminally tagged with a 2 × HA-tag under the control of the GAL1 promoter | A. Schmidt |
| pJK701 | HA-SLM1 in pAS24 | This study |
| pJK702 | HA-SLM1 in pAS25 | This study |
| pSW1 | HA-SLM2 in pAS24 | This study |
| pJK703 | HA-SLM2 in pAS25 | This study |
| pJK704 | HA-SLM1ΔC in pAS25 | This study |
| pJK705 | HA-SLM2ΔC in pAS25 | This study |
| pSW2 | HA-SLM1ΔC in pAS24 | This study |
| PSW4 | slm1–3 in p416GPD, CEN, URA3 | This study |
| PSW5 | slm1–3 in p415GPD, CEN, LEU2 | This study |
| pAD41 | HA-SLM1M1 in pAS25 | This study |
| pAD42 | HA-SLM1M2 in pAS25 | This study |
| pAD43 | HA-SLM2M1 in pAS25 | This study |
| PJK706 | SLM1-PH in pGEX5 × –1 | This study |
| pAD44 | GST-SLM1-PHM1 in pGEX5 × –1 | This study |
| pAD45 | GST-SLM1-PHM2 in pGEX5 × –1 | This study |
| pJK708 | 6HIS-SLM1 in pET28a (Novagen) | This study |
| pJK709 | 6HIS-SLM2 in pET28a (Novagen) | This study |
| pJK710 | AVO2 in TNT521 (Udan et al., 2003) | This study |
| pJK711 | BIT61 in TNT521 | This study |
| pJK712 | YBR270C in TNT521 | This study |
| pC-186 | Genomic fragment containing the RHO1 gene, 2μ URA3 | Madaule et al. (1987) |
| pRHO2 | Genomic fragment containing the RHO2 gene in Yeplac195, 2μ ARS, URA3 | M. Hall |
| pPKC1 | Genomic fragment containing PKC1 in Yeplac195, 2μ ARS, URA3 | M. Hall |
| pPKC1(R398P) | Ycp50, URA3, CEN containing the dominant active allele PKC1(R398P) | Levin et al. (1990) |
| pBCK1 | Genomic fragment containing BCK1 in pRS314, TRP1, CEN | Lee and Levin (1992) |
| pBCK1-20 | Genomic fragment containing the dominant active allele BCK1–20 in pRS314, TRP1, CEN | Lee and Levin (1992) |
| pDLB824 | CEN HA-MKK1DD; constitutively activated MKK1-DD mutant, altering residues Ser-377 and Thr-381 to Asp | Harrison et al. (2004) |
| pMPK1 | Genomic fragment containing HA-MPK1 in Ycp50 (CEN, URA3) | M. Gustin |
| pHA-TOR2 | 2 × HA-tagged TOR2 in pAS25 | Kunz et al. (2000) |
| pHA-TOR2-KD | 2 × HA-tagged TOR2 kinase-dead mutant in pAS25 | Kunz et al. (2000) |
| PGAL1-SigD | SigD ORF under control of the GAL1 promoter in pTB227 (URA3, CEN; Kunz et al., 2000) | This study |