Figure 7.
Id2 inhibits BMP-7 from regulating α-SMA expression. (A and B) Semiquantitative RT-PCR analysis showing the mRNA steady state levels of Idb2 and control Gapdh. NMuMG cells were treated with vehicle (-), 5 ng/ml TGF-β1 for 2, 8, and 36 h (A) or 300 ng/ml BMP-7 (+) for 2, 8, and 48 h (B). Water and minus RT controls show the specificity of the reactions. (C) Knockdown of Id2 allows BMP-7 to cause EMT and enhance α-SMA protein synthesis in a time-dependent manner. NMe clonal cells were transfected with control (siRNA-luc) or specific (siRNA-Id2) and then treated with vehicle (0) or with 300 ng/ml BMP-7 for 8 or 36 h. The levels of endogenous α-SMA, Id2, and β-tubulin were determined by immunoblotting.