Figure 9.

eng1Δ agn1Δ cells are defective in cell separation. Morphology of ace2Δ (LE25, A) and eng1Δ agn1Δ (YAB54, B) cells. (C–E) Magnifications of representative groups of ace2Δ (D) and eng1Δ agn1Δ (E) cells, with the wild-type (C) as control. Cells were grown in rich medium to early-log phase, fixed with ethanol, and stained with aniline blue and DAPI. eng1Δ agn1Δ mutants show a mycelial and branched phenotype, similar to ace2Δ mutants, although the cells are less elongated. Scale bar, 10 μm.