SNX9 interacts directly with dynamin in vitro. The GST fusion proteins, GST-SNX9, GST-SNX9ΔSH3, GST-SH3(SNX9), or GST alone were immobilized on glutathione-Sepharose-beads and incubated with purified dynamin-1, dynamin-1ΔPRD, dynamin-2, or dynamin-2ΔPRD as described in Materials and Methods. Bound proteins were then analyzed by SDS-PAGE and Coomassie blue staining.