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Caveolin cycles between the PM and intracellular lipid bodies. Vero cells were transfected with Cav3-GFP for 24 h in the presence of 50 μg/ml oleic acid. After photobleaching of the cell except the PM, images were captured every 1 min for a total period of 60 min (Videos 5 and 6). Photobleached LBs recovered 21 ± 3 of the original fluorescence within 1 h (A and B), even in the presence of Cyhx (17 ± 1, C and D). Bar, 5 μm.
