Figure 3.

Golgi-associated caveolin predominantly corresponds to newly synthesized protein. BHK cells were incubated for different times with Cyhx (a general inhibitor of protein translation), and the Golgi-associated pool of caveolin was monitored by means of an anti-Go-cav antibody. The amount of caveolin present in the Golgi complex was quantified as percentage of pixel intensity with respect to control cells (G). A reduction to 59± 16 or 10 ± 8% in the amount of caveolin was detected after 1 h (B) or 3 h (C), respectively. Next, the cells were incubated for 3 h with Cyhx, and the drug was washed out, allowing the cells to recover. After 30 min at 37°C, 43 ± 12% of caveolin reappeared in a the Golgi complex (D). The recovery was completely blocked at 15°C (7 ± 1%) (F) but unaffected at 20°C (46 ± 14%) (E). Bar, 5 μm.