Figure 5.

Cholesterol modulates caveolin traffic through the Golgi complex. BHK cells were incubated for 2 h in a medium enriched in cholesterol (C), with CyHx (B) or with both (D). The Golgi-associated caveolin was monitored by means of anti-Go-cav antibody. In the presence of elevated cholesterol, the rate of decrease in the Golgi caveolin pool observed after treatment with Cyhx was accelerated from 25 ± 9 to 11 ± 3% (D and E for quantification). (F and G) Cells were incubated for 90 min with cholesterol or cyclodextrin, Cyhx, or both, and detergent-soluble caveolin was immunoprecipitated by anti-Go-cav antibody. Less caveolin was precipitated from cells treated with cholesterol. However, few changes in the amount of caveolin were observed when the Golgi exit was blocked at 20°C. (G) In contrast, more caveolin was immunoprecipitated from cells treated with 2% CD. Bar, 5 μm.