Figure 8.

(A–C) Caveolin cycle. (A) BHK cells were transfected with Cav3-GFP for 3 h. (B) Next, the transfection medium was replaced by a medium containing Cyhx, and the cells were incubated for an additional 3 h. (C) Finally, 50 μg/ml oleic acid was added for 6 h. (D and E) BHK cells were transfected with Cav3-GFP for 2 h in the presence of 5 μg/ml BFA. Next, the transfection medium was replaced by medium that contained Cyhx and BFA for an additional 2 h (D). Finally, BFA was washed out, and the cells were further incubated in a media containing Cyhx for 2 h (E). Bar, 5 μm.