FIG. 9.

Transcriptional mapping of the regR promoter region. Total RNA was purified from B. japonicum wild type grown aerobically in PSY medium and used for primer extension. Hybridization to RNA with the ³²P-labeled oligonucleotide PEregR2 and reverse transcription of the regR mRNA were performed as described in Materials and Methods. The products were separated on a 6% polyacrylamide gel next to a sequence ladder of plasmid pRJ2403 made with oligonucleotide PEregR2. The sequence of the indicated region (positions 1448 to 1520 of the database-submitted nucleotide sequence) is denoted in the box. It contains the transcription start point (+1), the putative −10 and −35 regions, and the probable translation start site (TTG).