TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Source or referencea |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 gyrA96 thi-1 relA1 | BRL |
| S17-1 | Smr SprhsdR (RP4-2 kan::Tn7 tet::Mu chromosomally integrated) | 55 |
| MC1061 | Δ(lacIPOZYA)X74 hsdR | 10 |
| UK198 | Nxrdam dcm | 23 |
| B. japonicum strainsb | ||
| 110spc4 | Spr wild type | 48 |
| A9 | Spr KmrnifA::aphII | 17 |
| 2408 | Spr SmrregS::Ω (same orientation) | This work |
| 2409 | Spr SmrregS::Ω (opposite orientation) | This work |
| 2408R | Spr Smr KmrfixR′-′lacZ regS::Ω (same orientation) | This work |
| 2409R | Spr Smr KmrfixR′-′lacZ regS::Ω (opposite orientation) | This work |
| 2410 | Spr KmrregS::aphII (same orientation) | This work |
| 2411 | Spr KmrregS::aphII (opposite orientation) | This work |
| 2426 | Spr SmrregR::Ω (opposite orientation) | This work |
| 2427 | Spr SmrregR::Ω (same orientation) | This work |
| 2426R | Spr Smr KmrfixR′-′lacZ regR::Ω (opposite orientation) | This work |
| 2427R | Spr Smr KmrfixR′-′lacZ regR::Ω (same orientation) | This work |
| 2428 | Spr KmrregR::aphII (same orientation) | This work |
| 2429 | Spr KmrregR::aphII (opposite orientation) | This work |
| 7276B | Spr KmrfixR′-′lacZ, chromosomally integrated | 57 |
| 7277C | Spr Kmr ΔUAS-fixR′-′lacZ, chromosomally integrated | 57 |
| Plasmids | ||
| pBluescript(SK+) | Apr, cloning vector | Stratagene |
| pUC18, pUC19 | Apr, cloning vectors | 41 |
| pUR2 | Apr, cloning vector | 49 |
| pSUP202 | Apr Cmr TcroriT from RP4 | 55 |
| pSUP202pol4 | Tcr (pSUP202) | 18 |
| pUC4-KIXX-PSP | Apr Kmr (pUC4-KIXX) aphII cassette with PmeI-SwaI-PacI linker in SmaI site | 31 |
| pHP45Ω | Apr Spr Smr (Ω cassette) | 46 |
| pSP72::regA | Apr (pSP72) R. capsulatus regA on an 857-bp BamHI-SalI fragment | 25 |
| pRJ2400 | Apr [pBluescript(SK+)] B. japonicum regSR on a 2.5-kb EcoRI-BamHI fragment | This work |
| pRJ2403 | Apr (pUC19) B. japonicum regSR on a 3.6-kb EcoRI fragment | This work |
| pRJ2408 | Spr Smr Tcr (pSUP202pol4) regS::Ω (same orientation) | This work |
| pRJ2409 | Spr Smr Tcr (pSUP202pol4) regS::Ω (opposite orientation) | This work |
| pRJ2410 | Kmr Apr Tcr (pSUP202pol4) regS::aphII (same orientation) | This work |
| pRJ2411 | Kmr Apr Tcr (pSUP202pol4) regS::aphII (opposite orientation) | This work |
| pRJ2426 | Spr Smr Apr Tcr (pSUP202) regR::Ω (opposite orientation) | This work |
| pRJ2427 | Spr Smr Apr Tcr (pSUP202) regR::Ω (same orientation) | This work |
| pRJ2428 | Kmr Apr Tcr (pSUP202) regR::aphII (same orientation) | This work |
| pRJ2429 | Kmr Apr Tcr (pSUP202) regR::aphII (opposite orientation) | This work |
| pRJ7211 | AprfixR′-′lacZ | 58 |
a
BRL, Bethesda Research Laboratories, Inc., Gaithersburg, Md.; Stratagene, La Jolla, Calif.
b
The genetic structure of the regS and regR mutant strains is depicted in Fig. 3C.