TABLE 3.
Relative heat shock mRNA levelsa
| Strain | Level of the following specific mRNAb:
|
|||
|---|---|---|---|---|
| hrcA | dnaK | dnaJ | hflB | |
| BK8 (dnaK+-erm) | 1.0 | 1.0 | 1.0 | 1.0 |
| BK11 (dnaKΔ2) | 3.0 | 3.2 | 1.2 | 1.1 |
| BK6 (dnaKΔ1) | 14 | 7.3 | 2.3 | 1.4 |
a
The strains were grown in GM17 supplemented with erythromycin at 30°C.
b
Northern blotting was performed with 10 μg of total RNA from each strain. Specific mRNA species were detected with radioactive double-stranded DNA probes covering the gene of interest. Radioactivity was measured in each lane with a Packard Instant Imager.