TABLE 5.

Comparison of PurBox sequences from L. lactis and B. subtilis

Operator sitea	Sequenceb	Locationc	Organism	Verification
PurBox C1	ATTTCCGAACATT	−93	L. lactis	Deletion
PurBox C2	AAAACCGAACAAT	−76	L. lactis	Deletion, mutation
PurBox C3	AAAACCGAACAAT	+263	L. lactis	None
PurBox D1	AATACCGAACAAT	−76	L. lactis	Deletion, mutation
PurBox D2	ATTTCCGAACTAT	+289	L. lactis	None
PurBox R1	ATTTCCGAACATT	−26R	L. lactis	None
Consensus sequence	AWWWCCGAACWWT
Nucleotide position	1.....7.....13
PurBox O	AAACACGAACATT	−75	B. subtilis	Binding studiesd
PurBox A	AAATCCGAACATT	−46R	B. subtilis	Binding studiese
PurBox R	AAATCCGAATATT	−20R	B. subtilis	Binding studiesf

^aPurR operator sites are labeled as follows: C1 to C3, purC; D1 and D2, purD; R1, purR; O, purine operon; A, purA; R, purR. 

^bGiven from the 5′ end and numbered from nucleotides 1 to 13. 

^cThe location of the PurBox is given for the G7 residue relative to the transcriptional start site. The notation −26^R indicates that the PurBox sequence is found at position −26 on the opposite strand. 

^dPurBox O has a putative core PurR binding site in front of the purine operon (8). 

^ePurBox A has a putative core PurR binding site in front of the purA gene (22, 35). 

^fPurBox R has a putative core PurR binding site in front of the purR gene (35, 39). The location of the PurBox is relative to the translational start site.