FIGURE 3.

Acute S100B exposure does not inhibit non-inactivating voltage-gated potassium currents (I_K) in midbrain neurons. (a) Representative traces of I_K from TH⁺ and TH⁻ neurons with (right) and without (left) 50 pM S100B. (b) Left panel, I–V curves of TH⁺ and TH⁻ neurons in regular aCSF. Right panels, average I–V curves from TH⁺ and TH⁻ neurons with and without S100B. (c) Comparison of max I_K from the most depolarizing stimulation step in TH⁺ and TH⁻ neurons. n = 11 for TH⁺ neurons and 12 for TH⁻ neurons from three independent weeks of culture. All errors are SEM; p-values for I–V curves are based on mixed-design ANOVA with post hoc Bonferroni correction; p-values for max I_K are based on paired sample t tests for TH⁺ control, TH⁺ S100B and TH⁻ control, TH⁻ S100B, and two sample t tests for TH⁺ control, TH⁻ control