FIGURE 5.

Acute exposure of primary midbrain cultures to S100B peptide increases spontaneous Ca²⁺ flux frequency only in TH⁺ neurons. (a) Multiple representative traces of spontaneous Ca²⁺ fluxes in TH⁺ and TH⁻ neurons with acute bath application of 50 pM S100B peptide are shown. (b) A graph with average frequency of Ca²⁺ flux events from individual TH⁺ (red) and TH⁻ (black) neurons with and without S100B peptide is shown. The line graph on the right shows the average fold change of Ca²⁺ flux frequency for TH⁺ and TH⁻ cells following S100B application. The graph below shows the average frequency of TH⁺ and TH⁻ neurons binned by week of culture. (c) A graph with average amplitude of Ca²⁺ events from individual TH⁺ (red) and TH⁻ (black) neurons with and without S100B peptide. The line graph on the right shows the average fold change of Ca²⁺ flux amplitude for TH⁺ and TH⁻ cells following S100B application. The graph below shows the average amplitude of TH⁺ and TH⁻ neurons binned by individual culture. n = 137 for TH⁺ neurons and 134 for TH⁻ neurons from eight independent cultures. All errors are SEM; p-values for all cells are based on Wilcoxon signed rank tests for TH⁺ spontaneous, TH⁺ S100B and TH⁻ spontaneous, TH⁻ S100B and Mann–Whitney tests for TH⁺ spontaneous, TH⁻ spontaneous; p-values for individual cultures are based on paired sample t tests for TH⁺ spontaneous, TH⁺ S100B and TH⁻ spontaneous, TH⁻ S100B, and two sample t tests for TH⁺ spontaneous, TH⁻ spontaneous