FIGURE 6.

Extracellular S100B mediated increase in spontaneous Ca²⁺ fluxes in TH⁺ DA neurons require active L-type VGCCs. (a) Representative traces of spontaneous Ca²⁺ fluxes in a TH⁺ and TH⁻ neuron with bath application of S100B, followed by co-application of S100B with diltiazem. (b) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁺ neurons without any drug (black) with bath applied S100B (green), and co-applied S100B + diltiazem (blue). (c) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁻ neurons without drug (black) with bath applied S100B (green), and co-applied S100B + diltiazem (blue). n = 55 for TH⁺ neurons and 57 for TH⁻ neurons from four independent weeks of culture. All errors are SEM; p-values for frequency and amplitude are based on Wilcoxon signed rank tests for all cases in panels b and c. (d) Representative traces of spontaneous Ca²⁺ fluxes in a TH⁺ and TH⁻ neuron with bath application of diltiazem, followed by co-application of diltiazem with S100B. (e) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁺ neurons without any drug (black) with bath applied diltiazem (blue), and co-applied diltiazem + S100B (green). (f) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁻ neurons without any drug (black) with bath applied diltiazem (blue), and co-applied diltiazem + S100B (green). n = 34 for TH⁺ neurons and 18 for TH⁻ neurons from four independent weeks of culture. All errors are SEM; p-values for frequency and amplitude are based on Wilcoxon signed rank tests for all cases in panels e and f