FIGURE 7.

Extracellular S100B mediated increase in spontaneous Ca²⁺ fluxes in TH⁺ DA neurons does not require active T-type VGCCs. (a) Representative traces of spontaneous Ca²⁺ fluxes in a TH⁺ and TH⁻ neuron after 15 min incubation with 1 μM mibefradil, followed by co-application of S100B with mibefradil. (b) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁺ neurons without any drug (black) with bath applied mibefradil (blue), and co-applied S100B + mibefradil (green). (c) Graphs show average Ca²⁺ flux frequency and amplitude of TH⁻ neurons without drug (black) with bath applied mibefradil (blue), and co-applied S100B + mibefradil (green). n = 75 for TH⁺ neurons and 66 for TH⁻ neurons from four independent weeks of culture. All errors are SEM; p-values for frequency and amplitude are based on Wilcoxon signed rank tests for all cases in panels b and c