FIG. 3.

Core enzyme plus ς^A is sufficient for in vitro transcription of the tagA and tagD promoters. Reactions and sample analysis were as described for Fig. 1. RNA markers are not shown. P, PhoP; R, PhoR; RNAP, purified B. subtilis RNA polymerase ς^A holoenzyme; E, isolated core enzyme; ς^A, purified ς^A. −, absent; +, present.