FIG. 5.

Primer extension analysis of the tuaA promoter in vivo and in vitro. The end-labeled primer FMH359 was annealed to RNA and then extended with avian myeloblastosis virus reverse transcriptase. Lane 1, RNA isolated from late-exponential-phase cells grown in LPDM; lane 2, RNA synthesized from the in vitro transcription reaction mixture containing PhoP∼P (products of the primer extension reactions are marked by an arrow); lanes A, G, C, and T are a sequencing ladder generated by annealing the same end-labeled primer to a plasmid, pES69, containing the tuaA promoter region (13) and extending it with Sequenase. The sequence of the region is indicated on the left. The asterisk indicates the base to which the primer extension product maps.