TABLE 1.
Accumulation of isoprimeverose
| Reaction no. | Uptake of isoprimeverose with protocol involvinga:
|
Intracellular glucose concn (mM) inc:
|
|||||
|---|---|---|---|---|---|---|---|
| Uptake treatment with:
|
Postuptake treatment with α-xylosidaseb |
L. plantarum 80/pLPA6
|
L. plantarum 80 (wild type)
|
||||
| l-Malate, 50 mM | Isoprimeverose, 0.5 mM | At 2 min | At 10 min | At 2 min | At 10 min | ||
| 1 | − | + | + | <0.2 | <0.2 | <0.2 | <0.2 |
| 2 | + | + | + | 2.01 ± 0.02 | 5.00 ± 0.25 | <0.2 | <0.2 |
| 3 | + | + | − | <0.2 | <0.2 | <0.2 | <0.2 |
a
+, uptake occurred; −, uptake did not occur. All experiments were performed in duplicate.
b
Corresponding to 20 μg of xylose-grown L. pentosus MD353 membrane protein.
c
The transport reaction was stopped at 2 min and at 10 min. All experiments were performed in duplicate.