Fig. 1.

Rim21 receives N-glycosylation at N15 residue. (a) Amino acid sequence of Rim21. The C-terminal cytosolic region is underlined. Sequences that match the potential conventional and unconventional N-glycosylation motifs are boxed. Predicted transmembrane segments are highlighted. (b) YOK2027 (rim21Δ) cells harboring an expression vector for WT or the mutant Rim21-HA were grown to the log phase, and their total lysates were prepared. The total lysates were then treated with or without Endo H and subjected to immunoblot analysis with the anti-HA antibody.