Fig. 3.
Rim21-N15Q is turned over more slowly than WT Rim21. (a) YOK2027 (rim21Δ) cells expressing Rim21-HA or Rim21-N15Q-HA were grown to the log phase and mock- or alkali-treated for 20 min. Cells were then treated with 200 μg/ml cycloheximide for the indicated periods and harvested. Their total lysates were subjected to immunoblot analysis with anti-HA and anti-Pgk1 antibodies. (b) Rim21-HA and Rim21-N15Q-HA levels were measured, normalized with those of Pgk1, and shown as a relative value to those at 0 min. Values represent the mean ± SD from three independent experiments.