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. 2019 Nov 30;45(1):1–8. doi: 10.1247/csf.19021

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Copyright 2020 The Author(s)

This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.

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Fig. 3 — Rim21-N15Q is turned over more slowly than WT Rim21. (a) YOK2027 (rim21Δ) cells expressing Rim21-HA or Rim21-N15Q-HA were grown to the log phase and mock- or alkali-treated for 20 min. Cells were then treated with 200 μg/ml cycloheximide for the indicated periods and harvested. Their total lysates were subjected to immunoblot analysis with anti-HA and anti-Pgk1 antibodies. (b) Rim21-HA and Rim21-N15Q-HA levels were measured, normalized with those of Pgk1, and shown as a relative value to those at 0 min. Values represent the mean ± SD from three independent experiments.