Table II.

Examples of studies using centrinone and human cells

Cell	Approach	Main finding	Reference
BT549, Calu-6, HCT116, HeLa, MDA-MB-231, RPE-1, U2OS	Centrinone	Centrosome loss leads to G1-phase cell cycle arrest in normal cells, but not in cancer cells.	Wong et al., 2015
SV589 HeLa	Centrinone	The link between the Golgi apparatus and centrosomes must be dissolved to reach metaphase.	Guizzunti and Seemann, 2016
RPE-1	Centrinone	A single Golgi can be maintained in the absence of centrosomes.	Wu et al., 2016
RPE-1	Centrinone CRISPR/Cas9 screen	The 53BP1-USP28 module induces G1-phase cell cycle arrest after centrosome loss.	Meitinger et al., 2016
HEK293T	Centrinone BioID	The interactions of centriolar satellite proteins are not affected by centrosome loss.	Gheiratmand et al., 2019
RPE-1	Centrinone	Centrosomes indirectly regulate k-fiber plus-ends via spindle length-dependent accumulation of HURP.	Dudka et al., 2019
A549, DU145, GI-1, HCT116, HeLa, MCF-7, PANC-1, SKOV-3, U2OS	Centrinone	NuMA-mediated pathways promote spindle bipolarity independently of centrosomes.	Chinen et al., 2020