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Effects of LPS-deficient recipients on complementation ability of F and R100 traN
| Plasmid | traN genotype | Mating efficiencya
|
|
|---|---|---|---|
| rfaP | Wild type | ||
| pOX38::Tc | F | 6 | 80 |
| R100-1 | R100-1 | 188 | 200 |
| pOX38N1::CAT/pKI375 | F | 4.7 | 130 |
| pOX38N1::CAT/pBK8-2818 | R100-1 | 31 | 73 |
| pOX38N1::CAT/pBS KS+ | Vector control | 0.0024 | 0.00023 |
Defined as in Table 2, footnote a. The rfaP mutant is strain CS2193. The wild-type strain is CS2198, which is rfaJ19::TnlacZ (Kmr) but does not affect mating efficiency.
