Fig. 1.
Validation of differentially regulated miRNAs in HCM mouse models by Taqman qPCR analysis. Total RNA was isolated, reverse transcribed and qPCR was performed with specific Taqman miRNA assays (Applied Biosystems). Relative quantity of each miRNA was calculated using the 2-ΔΔCT method (snoRNA202 used as a normalization control). miRNA expression levels were quantified for the following mice models: (A) HCM_CsA vs. WT_CsA mice (n = 3–4); (B) HCM_Aged vs. WT_Aged mice (n = 3); (C) Mybpc3 homozygous mutant (MyBPCt/tvs. WT mice (n = 3). Data are represented as geometric mean of relative quantity ±SD, Statistics: 2way ANOVA with Sidak's multiple comparisons test. Adjusted p value **** p < 0.0001, ***p < 0.001, ** p = 0.0085, *p < 0.05.