Fig. 2.

miR-199a-3p mimic expression regulates expression of ECM. (A) Adult murine cardiac fibroblasts were transfected with control or miR-199a-3p mimics and conditioned media was collected and processed for mass-spectrometric analysis. Volcano plots depicting proteomics analysis in basal unstimulated conditions (left graph) and in TGFβ1 stimulated conditions (right graph). Blue dots indicate significantly down-regulated proteins and red dots indicate significantly up-regulated proteins in miR-199a-3p mimic expressing cells as compared to control-mimic expressing cells (B) Validation of proteomics data was conducted by corresponding gene expression levels through quantitative PCR. Relative quantity of each gene was calculated using the 2^-ΔΔCT method (Tbp used as a normalization control). Data are represented as geometric mean ± SD (n = 4 experiments). Statistics: Paired 2-tailed t-test with multiple comparisons-FDR, p value **p < 0.01, ***p < 0.001, ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)