Fig. 3.

Effects of in vivo miR-199a-3p inhibition on echocardiography and gravimetric parameters of HCM mice. HCM mice were treated with either LNA-control (n = 9, blue-filled circles or LNA-antimiR-199a-3p (n = 7, red-filled circles) (12.5 mg/kg monthly). (A) diastolic left ventricular posterior wall thickness (LVPW;d) (B) diastolic left ventricular internal dimension (LVID;d) and (C) fractional shortening (FS) % at baseline and end-point of the study. Data are expressed as mean ± SEM. Statistics: 2way ANOVA with Sidak's multiple comparisons test. P value: *p = 0.0457, **p = 0.0082, ****p < 0.0001. (D) Isovolumetric relaxation time (IVRT) at end-point. (E) Gravimetric analysis shows left atrial /tibia length ratio (LA/TL). Data are expressed as mean ± SEM, Statistics: Unpaired two-tailed t-test. P value p = ns (not significant). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)