Fig. 5.

Effects of in vivo miR-199a-3p inhibition on cardiac remodeling in HCM mice. (A) Micrographs depict 5 μm paraffin-embedded heart sections from LNA-control and LNA-antimiR-199a-3p treated mice stained with Masson's Trichrome (MT). Blue staining depicts collagen deposition areas. Scale bar = 0.5 mm (B) Graph shows quantification of fibrosis in heart sections using NIH Image J Software in LNA-control (blue-filled circles) and LNA-antimiR-199a-3p (red-filled circles) treated mice hearts. Data are expressed as mean ± SEM. Statistics: Unpaired 2-tailed t-test with Welch's correction, *p-value <0.05. mRNA levels of collagens (C) Col1a1 and (D) Col3a1 were quantified by real time-quantitative PCR in left ventricular heart tissue obtained from LNA-control (n = 9, blue filled circles) or LNA-antimiR-199a-3p treated HCM mice (n = 7, red filled circles). Relative quantity of each gene was calculated using the 2^-DDCT method (Tbp was used as a normalization control). Data are expressed as geometric mean ± SD. Statistics: Unpaired 2-tailed t-test with Welch's correction. P value * p < 0.05 (E) Representative immunoblots show Periostin and Gapdh protein expression (left) and densitometric analysis of Periostin normalized to Gapdh levels (LNA-control n = 9 or LNA-antimiR-199a-3p treated n = 7 HCM mice). Data are presented mean ± SEM. Statistics: Unpaired Mann-Whitney test. P value ** p = 0.005. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)