Fig. 6.

Identification of direct targets of miR-199a-3p in cultured adult mouse cardiac fibroblasts. mRNA levels of computationally predicted miR-199a-3p targets were quantified by real time-quantitative PCR in cardiac fibroblasts, cultured in serum-free media or 10 % FBS/DMEM, transfected with either control mimic (cntr) or miR-199a-3p mimic (199a-3p). (A) Cd151, (B) Itga3, (C) Rgs4, (D) Adamtsl3, (E) Cited2, (F) Rb1 (G) Kdm6a (H) Pnrc1 (I) Dnmt3a (J) Mkrn1 (K) mTor (L) Egr1, (M) Sbno2, (N) Nid2, (O) Itga8 (P) Dnmt1. Relative quantity of each gene was calculated using the 2^-DDCT method (Hprt was used as a normalization control, n = 4 per group). Data are presented geometric mean ± SD. Statistical analysis was performed by two-way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.