Figure 1.

(A) Schematic of Ago2 loaded with a short RNA guide sequence, binding to a target site using a seed driven approach. (B) Schematic of a chimeric read containing fragments of the small RNA guide sequence and the target site. (C) Experimental outline of the CLASH technique. (D) Outline of the bioinformatic pipeline for identification of single genomic, single small RNA, and chimeric reads. (All schematics in this figure were produced using Adobe Illustrator 2023 v27.9).