TABLE 1.
NADH dehydrogenase activity in mitochondrial extracts from parental and NDH disruption strains
| Relevant genotype | Mitochondrial NADH dehydrogenase activity (μmol/min/mg)
|
|
|---|---|---|
| Glucosea | Glycerol-lactate | |
| Parental | 0.48 ± 0.12b | 1.49 ± 0.22 |
| ΔNDH1 | 0.18 ± 0.07 | 1.59 ± 0.11 |
| ΔNDH2 | 0.63 ± 0.24 | 1.24 ± 0.22 |
| ΔNDH1 ΔNDH2 | 0.22 ± 0.01 | 1.48 ± 0.45 |
a
Yeast strains were cultivated on rich medium with the indicated carbon source prior to cellular fractionation.
b
Values represent averages and standard deviations of two or three independent measurements.