TABLE 2.
Rates of oxygen consumption of isolated mitochondria from parental and NDH disruption strains
| Relevant genotype | Oxygen consumption (μmol/mg of protein/min)
|
Ratio of:
|
|||
|---|---|---|---|---|---|
| NADHa | Succinate | Pyruvate + malate | NADH/succinate | NADH/pyruvate + malate | |
| Parental | 0.25 ± 0.08b | 0.06 ± 0.02 | 0.14 ± 0.03 | 4.2 | 2.5 |
| ΔNDH1 | 0.08 ± 0.04 | 0.07 ± 0.03 | 0.10 ± 0.02 | 1.1 | 0.8 |
| ΔNDH2 | 0.23 ± 0.05 | 0.06 ± 0.01 | 0.13 ± 0.03 | 3.8 | 2.2 |
| ΔNDH1 ΔNDH2 | 0.07 ± 0.02 | 0.06 ± 0.01 | 0.08 ± 0.01 | 1.2 | 0.9 |
a
Polarographic measurements were conducted as described in Materials and Methods by using mitochondria isolated from cells grown in YP glucose medium.
b
Values represent averages and standard deviations of two or three independent measurements.