TABLE 4.
Enzyme activities in extracts from parental and disruption mutant strains
| Relevant genotype | Activity (μmol/min/mg) of:
|
||
|---|---|---|---|
| NADH dehydrogenase in mitochondriaa | Malate dehydrogenase in:
|
||
| Mitochondriab | Cytosol | ||
| Parental | 0.48 ± 0.12c | 1.85 ± 0.12 | 0.27 ± 0.09 |
| ΔNDH1 | 0.18 ± 0.07 | 2.16 ± 0.04 | 0.22 ± 0.04 |
| ΔMDH1 | 0.39 ± 0.06 | 0.38 ± 0.09 | 0.28 ± 0.15 |
| ΔNDH1 ΔMDH1 | 0.19 ± 0.06 | 0.45 ± 0.01 | 0.21 ± 0.01 |
| ΔMDH2 | 0.51 ± 0.02 | 1.93 ± 0.22 | 0.05 ± 0.01 |
| ΔNDH1 ΔMDH2 | 0.17 ± 0.01 | 1.45 ± 0.06 | 0.03 ± 0.01 |
a
Subcellular fractionation was conducted as described in Materials and Methods by using cells cultivated on YP glucose medium.
b
Subcellular fractionation was conducted by using cells cultivated on YP glycerol-lactate.
c
Values represent averages and standard deviations of two or three independent measurements.