TABLE 5.

Free amino acids in bacterial strains^a

Strain	Medium	Intracellular concn (mM) ofb:
		Thr	Val	Leu	Ile	Met	2-Aminobutyrate
LT2	Glucose	0.08 ± 0.003	1.14 ± 0.01	0.72 ± 0.27	0.17 ± 0.04	0.070 ± 0.006	ND
TV105	Glucose	—	1.56	0.65	0.46	0.11	ND
TV496	Glucose + pantothenate	ND	1.79	1.42	0.37	0.08	0.7
TV496	Glucosec	ND	2.37	0.52	0.11	0.07	0.3
TV497	Glucose + pantothenate	0.05 ± 0.012	1.08 ± 0.32	1.12 ± 0.05	5.90 ± 0.43	0.054 ± 0.010	0.50 ± 0.03
MM294	Glucose	0.29	3.7	1.08	0.31	0.59	0.1
LT2	Acetate	0.03	3.76	0.26	0.19	0.11	ND

^aThe amino acid analysis was performed as described in Materials and Methods. 

^bBacteria were grown to the steady state, rapidly filtered, and extracted with boiling water as described in the text. Intracellular concentrations were calculated from the results of automated amino acid analysis with a slow gradient (see Results). Data shown for LT2 and TV497 in glucose medium are the averages of two independent analyses. ND, not detected (the limits of detection were apparently ≤0.02 mM). —, peaks of threonine and serine were too close for a reliable estimate of the threonine concentration in this sample. 

^cTV496 does not attain a constant growth rate in glucose minimal medium without additions. An aliquot of a culture grown to the steady state with the addition of pantothenate was diluted 10-fold to an absorbance of 4 Klett units in glucose minimal medium and allowed to double three times (40 Klett units) before being harvested for amino acid analysis.