TABLE 4.
Presence of cross-hybridizing DNA in other β-lactam-producing actinomycetes
| Actinomycetea | β-Lactam antibiotic(s) | Extent of hybridizationb
|
||||
|---|---|---|---|---|---|---|
| lat | ccaR | orf11 | blp | pcd | ||
| S. clavuligerus | Cephamycin C, clavams, clavulanic acid | +++ | +++ | +++ | +++ | +++ |
| S. cattleya | Cephamycin C | ++ | + | − | − | ++ |
| S. griseus | Cephamycins A and B | ++ | + | − | − | ++ |
| S. jumonjinesis | Cephamycin C, clavulanic acid | + | + | + | − | + |
| S. lipmanii | 7-Methoxycephalosporin C, clavaminic acid | ++ | + | ++ | − | +++ |
| N. lactamdurans | Cephamycin C | ++ | − | − | − | − |
a
A combination of β-lactam-producing actinomycetes (S. clavuligerus NRRL 3585, S. cattleya NRRL 3841, S. griseus NRRL 3851, S. jumonjinensis NRRL 5741, S. lipmanii NRRL 3584, and N. lactamdurans NRRL 3802) was used.
b
Qualitative score relative to the hybridization to the S. clavuligerus DNA, indicated as strong hybridization (+++), moderate hybridization (++), weak or very weak hybridization (+), and no hybridization (−).