Fig. 3. UBE2O interacts with IFIT3.

A Immunoprecipitation was carried out using anti-rabbit IgG and an anti-UBE2O antibody. Following electrophoresis, the gel strips were stained with Coomassie blue. B The indicated cells were lysed in buffer and then subjected to Co-IP analysis with protein A/G magnetic beads and anti-UBE2O or anti-IFIT3 antibodies, followed by Western blot analysis (n = 3). C 293 T cells were transfected with plasmids containing different tags. After 48 h, the cells were treated with MG132 (10 μM) for 4-6 h. Cells were lysed with buffer and then subjected to Co-IP analysis with protein A/G beads and antibodies specific for the corresponding tags, followed by Western blot analysis (n = 3). D Truncation mutants of IFIT3 and UBE2O were generated as indicated, and plasmids carrying different tags were transfected into 293 T cells. IP was performed using anti-HA and anti-Myc antibodies, followed by IB with the corresponding antibodies.