Table 1.

K_m and V_max of AgaB, AgaB mutants and truncations.

	Km (mg/mL)	Vmax (U/mg)
AgaBa	3.18 ± 0.23	0.24 ± 0.13
AgaBb	0.35 ± 0.01	0.63 ± 0.11
Truncation-2	4.30 ± 0.37	0.79 ± 0.04
Truncation-3	5.47 ± 0.28	3.96 ± 0.30
E468Q	ND	ND
D259N	0.11 ± 0.01	0.55 ± 0.03
D333N	ND	ND
E452Q	0.27 ± 0.02	1.21 ± 0.05

^aAgaB activity was detected in 20 mM Tri-HCl buffer without Ca²⁺.

^bAgaB activity was detected in 20 mM Tri-HCl buffer without 10 mM Ca²⁺.