Table 2.

Primers and TaqMan probes for qPCR of pathogenic Leptospira spp.

Target gene	Nucleotide sequence	Reference
LipL32(1)
Forward	5′-AAG CAT TAC CGC TTG TGG TG-3′	36
Reverse	5′-GAA CTC CCA TTT CAG CGA TT-3′
TaqMan probe	FAM/ZEN-5’-AA AGC CAG GAC AAG CGC CG-3’-IBFQ
LipL32(2)
Forward	5′-CGG GAG GCA GCA GTT AAG AAT-3′	41
Reverse	5′-ACG TAT GGT GCA AGC GTT GTT-3′
TaqMan probe	FAM/ZEN-5′-GCA ATG TGA TGA TGG TAC CTG CCT-3′-IBFQ