Table 3.

Comparison of Ct values between a qPCR assay with the LipL32(1) primers and one with the LipL32(2) primers

Group	DNA concentration (ng/μL)	LipL32(1) Ct value	LipL32(2) Ct value
Positive control
L. borgpetersenii serovar Ballum strain Mus 127	26.4	15.33	32.08
L. borgpetersenii serovar Hardjobovis strain 117123	20.6	17.41	33.36
L. borgpetersenii serovar Javanica*	8.2	16.22	36.14
L. borgpetersenii serovar Javanica strain Veldrat Bataviae 46	41.0	14.35	33.96
L. borgpetersenii serovar Tarassovi strain Perepelitsin	16.1	20.54	36.39
L. interrogans serovar Australis*	9.9–11.9	15.43–15.58	33.78–34.38
L. interrogans serovar Australis strain Ballico	11.8	29.67	N
L. interrogans serovar Autumnalis strain Akiyami A	32.6	23.56	N
L. interrogans serovar Bataviae*	5.3–20.1	13.91–16.02	34.11–35.91
L. interrogans serovar Bataviae strain Swart	15.8	17.97	34.29
L. interrogans serovar Canicola strain Hond Utrecht IV	12.7	14.99	34.08
L. interrogans serovar Copenhageni strain M20	2.5	17.95	34.44
L. interrogans serovar Djasiman strain Djasiman	11.5	16.20	35.43
L. interrogans serovar Hebdomadis strain Hebdomadis	28.4	16.77	33.91
L. interrogans serovar Icterohaemorrhagiae strain RGA	16.8	14.3	32.81
L. interrogans serovar Lai strain Lai	12.8	19.22	36.19
L. interrogans serovar Pomona strain Pomona	7.2	18.05	35.44
L. interrogans serovar Pyrogenes strain Salinem	43.3	21.40	N
L. kirschneri serovar Cynopteri strain 3522C	29.7	14.44	33.60
L. kirschneri serovar Grippotyphosa strain Moskva V	42.3	13.04	31.79
L. kmetyi serovar Malaysia strain Bejo-ISO9	36.1	18.78	33.34
L. weilii serovar Celledoni strain Celledoni	13.0	24.15	40.52
Non-target negative control
Bacillus spp.	9.9	N	N
Escherichia coli	16.0	N	N
L. biflexa serovar Patoc strain Patoc I	42.9	N	N
L. selangorensis **	8.7-14.3	N	N
L. wolffii**	6.7-19.3	N	N
Pseudomonas aeruginosa	84.9	N	N
Salmonella spp.	41.1	N	N
Staphylococcus aureus	14.4	N	N
Streptococcus spp.	4.2	N	N
No-target negative control
RNase-free water	0	N	N

¹* - dog isolates;

¹** - environmental isolates; N - negative