Table 4.
Threshold cycle (Ct) values of fivefold serial dilution of Leptospira genomic DNA in a 20 μL reaction volume
| Dilution factor | Mean DNA copy number/μL | log DNA copies | Mean Ct value |
|---|---|---|---|
| Standard control with DNA concentration of 51.85 ng/μL (primary stock) | 10,382,320 | 7.01629441 | 12.47 |
| 1:5 | 2,076,464 | 6.317324406 | 14.15 |
| 1:25 | 415,292.8 | 5.618354402 | 17.66 |
| 1:125 | 83,058.56 | 4.919384397 | 18.96 |
| 1:625 | 16,611.712 | 4.220414393 | 21.05 |
| 1:3,125 | 3,322.3424 | 3.521444389 | 23.36 |
| 1:15,625 | 664.46848 | 2.822474384 | 25.79 |
| 1:78,125 | 132.893696 | 2.12350438 | 27.43 |
| 1:390,625 | 26.5787392 | 1.424534376 | 29.67 |
| 1:1,953,125 | 5.31574784 | 0.725564371 | 32.00 |