. 2023 Dec 19;11:e16611. doi: 10.7717/peerj.16611

Table 1. The information of primers of the candidate genes and their relative expression.

Gene	Name	Primer (5′→3′)	Relative expression
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	AGATGGTGAAGGTCGGAGTG	/
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	CGTTCTCTGCCTTGACTGTG	/
LPL	Lipoprotein lipase	AGCTCCAAGTCGCCTTTCTC	0.47
LPL	Lipoprotein lipase	TCCTGGTTGGAAAGTGCCTC	0.47
LDLR	Low density lipoprotein receptor	TGTTGGACACACGTACCCAG	1.14^**
LDLR	Low density lipoprotein receptor	AAGGTCGCGACTTGTCTCAG	1.14^**
CD36	Platelet glycoprotein 4	GACGGATGTACAGCGGTGAT	3.32^*
CD36	Platelet glycoprotein 4	GAAAAAGTGCAAGGCCACCA	3.32^*
ACSL1	Acyl-CoA synthetase long-chain family member 1	AGCCGCATTTCACTTTTACTGC	0.49^**
ACSL1	Acyl-CoA synthetase long-chain family member 1	AGCTCTTTAGGGCAAACCCC	0.49^**
FABP3	Fatty acid-binding protein 3	ACGCGTTCTCTGTCGTCTTT	1.13
FABP3	Fatty acid-binding protein 3	AACCGACACCGAGTGACTTC	1.13
ACACA	Acetyl-coa carboxylase/biotin carboxylase 1	ACGGCTGACTGGAGTTGAAG	0.61
ACACA	Acetyl-coa carboxylase/biotin carboxylase 1	AACGTCTGCTTGTCCGTCTT	0.61
ACBP	Aacyl-coa-binding protein	TGGAATCTTTGCAACACCGC	0.77^**
ACBP	Aacyl-coa-binding protein	TGTCACCCACAGTTGCTTGT	0.77^**
FASN	Fatty acid synthase	CCTCAAGATGAAGGTGGTGCT	1.64^**
FASN	Fatty acid synthase	GGCCCTGGGTTATATCGAGC	1.64^**
SCD	Stearoyl-CoA desaturase	TCCTGATCATTGGCAACACCA	0.83^*
SCD	Stearoyl-CoA desaturase	CCAACCCACGTGAGAGAAGAA	0.83^*
DGAT1	Diacylglycerol o-acyltransferase 1	TACCCCGACAACCTGACCTA	0.85^*
DGAT1	Diacylglycerol o-acyltransferase 1	GGGAAGTTGAGCTCGTAGCA	0.85^*
LPIN1	Lipin 1	CTTCGATTCCCAAACCGGGA	4.01^*
LPIN1	Lipin 1	TCACAGTGACGAACACCTGG	4.01^*
ADFP	Perilipin-2	GCGTCTGCTGGCTGATTTC	1.16^**
(PLIN2)	Perilipin-2	AGCCGAGGAGACCAGATCATA	1.16^**
APOE	Apolipoprotein E	CGGTTTCTGGAGGCGAAGAA	0.68
APOE	Apolipoprotein E	CTCCATATCCGCCTGGCATC	0.68
PRKCA	Calcium-activated, phospholipid- and diacylglycerol- dependent serine	GACTTCGGGATGTGCAAGGA	0.96
PRKCA		CGTACGGCTGATAGGCGATT	0.96
MAPK1	Mitogen-activated protein kinase 1	AACAAAGTCCGAGTCGCCAT	0.72^**
MAPK1	Mitogen-activated protein kinase 1	CGATGGTCGGTGCTCGAATA	0.72^**
ARF6	ADP-ribosylation factor 6	AACTGGTATGTGTCAGCCCTC	0.56^*
ARF6	ADP-ribosylation factor 6	GAAAGAGGTGATGGTGGCGA	0.56^*
STAM1	Signal transducing adapter molecule 1	CCTGGTACTGCGGCTAACAA	0.59^**
STAM1	Signal transducing adapter molecule 1	ACGAACTTTCCGGCCTTCAT	0.59^**
EEA1	Early endosome antigen 1 isoform x4	CAGGCCCAGGACAGCTTAAA	0.93
EEA1	Early endosome antigen 1 isoform x4	GCAAGTTCCTGTGCTGCTTG	0.93
HERC3	HECT and RLD domain containing E3 ubiquitin protein ligase 3	CTCGAGGGCCTAGCTGTCT	0.92
HERC3		TTTGTCAGAAGGGTCTGGCG	0.92
VPS45	Vacuolar protein sorting 45 homolog	CCCCAAAGATGCTGTGGCTA	0.85^**
VPS45	Vacuolar protein sorting 45 homolog	AGTGTGCTGGGGCCTAGATA	0.85^**
CHMP2B	Charged multivesicular body protein 2b	ACGAGGTACACAGAGGGCTA	0.60^*
CHMP2B	Charged multivesicular body protein 2b	AGCTGTTTGGCTAAAACTCTGC	0.60^*
CHMP3	Charged multivesicular body protein 3	GTTTGAAATCACCGCAGGGG	0.64^*
CHMP3	Charged multivesicular body protein 3	CTAAAGGTTCAGGCTCCGGG	0.64^*
PIP5K	Phosphatidylinositol-4-phosphate 5-kinase, putative	CTCAGCACCTGGAAGAGCAA	0.72
PIP5K	Phosphatidylinositol-4-phosphate 5-kinase, putative	TTCTTCTTTCCCCGAGCCAC	0.72
CYHR1	Cysteine and histidine rich 1	GCCAACCTGCTTTTGGGAAG	0.72^*
CYHR1	Cysteine and histidine rich 1	GGTTGTGAAAACGGCCACAA	0.72^*
CP	Ubiquitin-like domain-containing CTD phosphatase 1	CATGGTGGCCAAAGGTGTTG	1.15
CP	Ubiquitin-like domain-containing CTD phosphatase 1	CATCTGCTGGAGATTTTTGGCA	1.15
PSMC1	proteasome 26S subunit, ATPase 1	GGTACGACTCCAACTCAGGC	0.89
PSMC1	proteasome 26S subunit, ATPase 1	ATCCGGTTTGTGGCCATGAT	0.89
PSMC3	26S protease regulatory subunit 6A	TGAACAAGACGCTGCCGTAT	0.87
PSMC3	26S protease regulatory subunit 6A	TGCCGCGTAGAGGTTTTGAT	0.87
PSMC5	26S proteasome regulatory subunit 8	CTCTGCACAAGATCCTGCCT	0.96
PSMC5	26S proteasome regulatory subunit 8	ATGCTTCACAGGCAGCTCAA	0.96
PSMD12	26S proteasome non-ATPase regulatory subunit 12	ATACGTCAGGCATCTCGCAG	1.04
PSMD12	26S proteasome non-ATPase regulatory subunit 12	GGCCATGTTGTAGGGGACAA	1.04
UBC	Ubiquitin-C	GGGAGGTGTTTTAAGTTCTCCCT	0.61
UBC	Ubiquitin-C	TTGAACTCTAACCCACCCCTAAC	0.61
UBA52	Ubiquitin-60S ribosomal protein L40	GCCCAGTGACACCATTGAGA	0.73^**
UBA52	Ubiquitin-60S ribosomal protein L40	GCAGGGTGGACTCTTTCTGG	0.73^**
UBA7	Ubiquitin-like modifier-activating enzyme 7	TCAGCAGGATGGTCTGAGGA	0.84^*
UBA7	Ubiquitin-like modifier-activating enzyme 7	AGTTCCAATACCAGCACCCG	0.84^*
TUBA	Tubulin beta chain	GTCTACTCCTGTTGCCTGC	0.77
TUBA	Tubulin beta chain	AGGCATTGCCGATCTGGAC	0.77
ISG15	Ubiquitin-like protein ISG15	CCATCCTGGTGAGGAACGAC	0.58^*
ISG15	Ubiquitin-like protein ISG15	GTCTGCTTGTACACGCTCCT	0.58^*
MX1	Interferon-induced GTP-binding protein Mx1	TGCCAACTAGTCAGCACTACATT	0.82^**
MX1	Interferon-induced GTP-binding protein Mx1	TGTACAGGTTGCTCTTGGACTC	0.82^**
SPP1	Secreted phosphoprotein 1	TCCGCCCTTCCAGTTAAACC	0.82
SPP1	Secreted phosphoprotein 1	GCTTCTGAGATGGGTCAGGC	0.82
RPS27A	Ribosomal protein S27a	TTTCGTGAAGACCCTGACGG	0.83^**
RPS27A	Ribosomal protein S27a	GTCTTTGCTGGTCAGGAGGAA	0.83^**

Notes:

Denotes P < 0.05.

^**

Denotes P < 0.01.