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Effects of aging on EMT and accumulation of collagen in the liver. (A,B) Immunohistochemical analysis of α-SMA expression level (20×). (C) Hydroxyproline content in liver tissue. (D) Changes in relative expression of COL1A1 and MMP9 genes. The results are presented as mean ± SEM, and the different letters (a,b) represent significant differences compared to 12 W (p < 0.05).
