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. 2023 Dec 12;10(12):1415. doi: 10.3390/bioengineering10121415

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Figure A2 — Tissue samples and histology. Legend: H&E staining of the fasciocutaneous flap skin after 24 h of continuous perfusion (1) and after 24 h of static storage at 21 °C (2). This photograph of H&E-stained tissue reveals the epidermis and partial dermis. The different cellular components can be visualized: epidermal stratum corneum (a), epidermal stratum spinosum (b), epidermal stratum basale (c), and dermis containing fibroblasts (d). The stratum spinosum and stratum basale in the control group (2) show minor apoptosis (black arrow). No necrosis is shown in any of the groups.